Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesThis product is an immunogold reagent built around colloidal gold particles of 6nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 12 %. The conventional labeling approach in transmission and scanning electron microscopy utilizes secondary immunogold reagents based on particles that can be observed without enhancement. This conjugate is suited for single and multiple labeling in electron microscopy, when the number of antigens available for binding is such that a relevant signal can be obtained.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notesElectron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 6nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also van Bergen en Henegouwen & Leunissen, Histochemistry, 85, (1986), 81.)

    Light Microscopy: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Guinea pig IgG H&L (ab37460)

    ab37460 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"