Overview

  • Product name
    Goat Anti-Human IgA H&L
    See all IgA secondary antibodies
  • Target species
    Human
  • Specificity
    Antibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • Tested applications
    Suitable for: ELISA, Dot blotmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose-bound human IgG and IgM. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • Clonality
    Polyclonal
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Dot blot
  • Application notes
    Recommended dilution as secondary antibody in ELISA is 1:1000.
    Recommended dilution as secondary antibody in dot-blotting is 1:100.
  • References for Goat Anti-Human IgA H&L (ab8499)

    ab8499 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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