Overview

  • Product nameGoat Anti-Human IgA H&L (FITC)
    See all IgA secondary antibodies
  • Target speciesHuman
  • SpecificityAntibodies were raised in goat to human IgA. There is low background fluorescence in normal tissue in contrast to cases of autoimmune desease where IgA is depostited. No cross reactivity with albumine fractions of human serum. No cross reactivity with albumine fractions of animal species.
  • Tested applicationsSuitable for: ICC/IFmore details
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage buffer50% glycerol, 0.1 M NaCl, 0.01 M sodium phosphate at pH 7.5.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesFrom the crude polyclonal the cross reactive antibodies were extracted by incubation with Sepharose-bound human IgM and IgG. Specific antibodies were absorpted by incubation with Sepharose-bound human IgA. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgA a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • ClonalityPolyclonal
  • General notesF/P ratio is 4-6 mol Fluorescein per 1 mol of goat IgA.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notesFor immunohistochemical staining of human tissue sections a 1:40 dilution is recommended.
  • References for Goat Anti-Human IgA H&L (FITC) (ab8500)

    ab8500 has not yet been referenced specifically in any publications.

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