Overview

  • Product name
    Goat Anti-Human IgE epsilon chain
    See all IgE secondary antibodies
  • Host species
    Goat
  • Target species
    Human
  • Specificity
    The affinity purified anti IgE reacts only with IgE ( several different IgE kappa and lambda myeloma proteins) by IEP and gel diffusion techniques.
  • Tested applications
    Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details
  • Immunogen

    Goats were immunized with a mixture of several highly purified human IgE myeloma proteins in Freund’s adjuvant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    0.01M Tris-0.14M sodium chloride pH 7-7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Serum was collected and passed through several non-IgE containing immunoaffinity supports to make the preparation delta chain specific. The anti delta was immunoaffinity purified off an immunoabsorbant containing a different IgE than was used for immunization thus minimizing idiotypic influences.
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

This product has been referenced in:
  • Vanshylla K  et al. Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Sci Rep 8:4244 (2018). Read more (PubMed: 29523808) »
  • Olivier CE  et al. In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization. Clinics (Sao Paulo) 67:1171-9 (2012). Read more (PubMed: 23070344) »

See all 2 Publications for this product

Publishing research using ab9159? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

4 reviews or Q&A

10
Votes: Highest First

What is the isotype of ab9159? Is it possible to obtain ~2 mg in PBS only (no sodium azide? Do you have any anti-human IgE abs, fab fragment, not rabbit host, immunogen affinity purified?

Thank you for your patience. ab9159 is a Goat IgG antibody. The specific isotype has not been determined. We can offer the antibody without azide, but the minimum order is 5 mg. Unfortunately we do not have any other anti-human IgE abs that...

Read More

Dear Sirs, I would like to use the below article for an ELISA to detect IgE. Can you let me know if it is cross-reactive with cynomolgus monkey IgE? Goat polyclonal Secondary Antibody to Human IgE - epsilon chain (ab9159) Best regards

Thank you for contacting us and for your interest in our products. I am sorry to confirm that as far as we are aware, ab9159 Goat polyclonal Secondary Antibody to Human IgE ] has never been tested with samples from cynomolgus monkey. All tested and ...

Read More

Can you tell me how this antibody performs in a Western Blot and the dilution range recommended by your company. Thanks

Thank you for your enquiry. This secondary antibody has been tested for application in Western blotting and I would recommend starting with a dilution of 1:1000 and optimize based upon results. Please note that our policy at Abcam is that if an anti...

Read More

I just have a short question. Do you have a goat polyclonal to Human IgE conjugated to AP or HRP in your catalog? We are working with allergenes and will set up Western Blotting. Is this anything you can help us with?

Thank you for your enquiry. We currently do not have a Human IgE polyclonal antibody that is conjugated to HRP or AP. We do have a monoclonal: ab7426 - Mouse monoclonal (GE-1) to Human IgE (alkaline phosphatase). If you have any more questions, please ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up