Goat Anti-Human IgG F(ab) (Alkaline Phosphatase) preadsorbed (ab49761)

Overview

  • Product nameGoat Anti-Human IgG F(ab) (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityThe conjugate is specific for IgG and IgG Fab fragment. The conjugate shows no reactivity with IgG Fc fragment.
  • Tested applicationsSuitable for: ELISA, IHC-P, Dot blotmore details
  • Minimal
    cross-reactivity

    Mouse, Rat more details
  • Immunogen

    Purified human IgG Fab fragment

  • Positive controlHuman tonsil tissue and Human IgG
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.05M Tris buffer, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab49761 is isolated from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG.
  • Conjugation notesab49761 is conjugated to Alkaline Phosphatase by protein cross linking with 0.2% glutaraldehyde.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
Dot blot
  • Application notesDot: 1/30000.
    ELISA: 1/40000.
    IHC-P: 1/50.
    Immunoblotting (chemiluminescent): 1/30000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Human IgG F(ab) (Alkaline Phosphatase) preadsorbed (ab49761)

    ab49761 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab49761.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"