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Product name Goat Anti-Human IgG F(ab) (Alkaline Phosphatase) preadsorbed See all IgG secondary antibodies
Target species Human
Specificity The conjugate is specific for IgG and IgG Fab fragment. The conjugate shows no reactivity with IgG Fc fragment.
Tested applications Suitable for:
ELISA, IHC-P, Dot blot more details
Minimal cross-reactivity Mouse, Rat
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Purified human IgG Fab fragment
Positive control Human tonsil tissue and Human IgG
Conjugation Alkaline Phosphatase Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.05M Tris buffer, 1mM Magnesium chloride, pH 8.0
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab49761 is isolated from goat anti-human IgG antiserum by immunospecific
purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG.
Conjugation notes ab49761 is conjugated to Alkaline Phosphatase by protein cross linking with 0.2% glutaraldehyde.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Dot: 1/30000.
Immunoblotting (chemiluminescent): 1/30000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Goat Anti-Human IgG F(ab) (Alkaline Phosphatase) preadsorbed (ab49761)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"