Goat Anti-Human IgG Fc (Alkaline Phosphatase) preadsorbed (ab50507)

Overview

  • Product nameGoat Anti-Human IgG Fc (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityThis antibody is specific for human IgG and human IgG Fc fragment. It shows no reactivity with human IgG Fab fragment, human light chains, human IgA or human IgM.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Dot blotmore details
  • Minimal
    cross-reactivity

    Mouse, Rat more details
  • Immunogen

    Purified human IgG, Fc fragment

  • Positive controlHuman tonsil sections and human IgG.
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.05M Tris buffer, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Conjugation notesAb50507 is conjugated to Alkaline Phosphatase by protein cross linking with 0.2% glutaraldehyde.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/40000. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent concentration.

Minimum titre 1/55000.

IHC-P 1/150.
Dot blot 1/40000.

References for Goat Anti-Human IgG Fc (Alkaline Phosphatase) preadsorbed (ab50507)

This product has been referenced in:
  • Boado RJ  et al. CHO cell expression, long-term stability, and primate pharmacokinetics and brain uptake of an IgG-paroxonase-1 fusion protein. Biotechnol Bioeng 108:186-96 (2011). ELISA . Read more (PubMed: 20803562) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. I can confirm that the concentration for this lot 757454 is 1.2 mg/ml. If there is anything else that I can help you with please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"