Goat Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed (ab7154)

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Overview

  • Product name
    Goat Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Human
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native human IgG (purified).

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.0095% Magnesium chloride, 50% Glycerol, 0.0014% Zinc chloride
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/1000 - 1/4000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Goat Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed (ab7154)

    ab7154 has not yet been referenced specifically in any publications.

    Publishing research using ab7154? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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