Goat Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed (ab7154)

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Overview

  • Product nameGoat Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native human IgG (purified).

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.79% Tris HCl, 0.0095% Magnesium chloride, 50% Glycerol, 0.0014% Zinc chloride
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • Application notesDot: Use at an assay dependant dilution.
    ELISA: 1/1000 - 1/4000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Goat Anti-Human IgG H&L (Alkaline Phosphatase) preadsorbed (ab7154)

    ab7154 has not yet been referenced specifically in any publications.

    Publishing research using ab7154? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"