Overview

  • Product name
    Goat Anti-Human IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Human IgG - H&L (Biotin), pre-adsorbed
  • Target species
    Human
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat more details
  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notes
    Biotin/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. ELISA-Primary
WB 1/10000 - 1/200000. WB-Chemiluminescent

References for Goat Anti-Human IgG H&L (Biotin) preadsorbed (ab97168)

This product has been referenced in:
  • Limberis MP  et al. Intranasal antibody gene transfer in mice and ferrets elicits broad protection against pandemic influenza. Sci Transl Med 5:187ra72 (2013). Human . Read more (PubMed: 23720583) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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