Overview

  • Product nameGoat Anti-Human IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Human IgG - H&L (Biotin), pre-adsorbed
  • Target speciesHuman
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat more details
  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • Conjugation notesBiotin/ antibody protein ratio is 4:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. ELISA-Primary
WB 1/10000 - 1/200000. WB-Chemiluminescent

References for Goat Anti-Human IgG H&L (Biotin) preadsorbed (ab97168)

This product has been referenced in:
  • Limberis MP  et al. Intranasal antibody gene transfer in mice and ferrets elicits broad protection against pandemic influenza. Sci Transl Med 5:187ra72 (2013). Human . Read more (PubMed: 23720583) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"