Goat Anti-Human IgG H&L (FITC) (ab8503)

Overview

  • Product nameGoat Anti-Human IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityAntibodies were raised in goat to human IgG, IgM and IgA
  • Tested applicationsSuitable for: Flow Cytmore details
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesSpecific antibodies were absorpted by incubation with Sepharose-bound human immuno- globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human immunoglobulins a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • ClonalityPolyclonal
  • General notesF/P ratio is 4-6 mol Fluorescein per 1 mol of goat Ig. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where Ig's are depostited.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.

References for Goat Anti-Human IgG H&L (FITC) (ab8503)

ab8503 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in FACS. All tested applications are specified on Abcam product datasheets. Please note that this antibody is not available to customers within the US due to import re...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"