Purification notesSpecific antibodies were absorpted by incubation with Sepharose-bound human immuno- globulins.
Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis.
After repeated binding with immobilized human immunoglobulins a minimum of 65% protein bound.
Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
General notesF/P ratio is 4-6 mol Fluorescein per 1 mol of goat Ig. There is low background fluorescence in normal tissue in contrast to cases of auto immune desease where Ig's are depostited.