• Product nameGoat Anti-Human IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Human IgG - H&L (HRP)
  • Target speciesHuman
  • Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    human IgG whole molecule

  • ConjugationHRP


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab6858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/5000 - 1/25000.

References for Goat Anti-Human IgG H&L (HRP) (ab6858)

This product has been referenced in:
  • Ku Z  et al. Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry. J Virol 89:12084-95 (2015). Read more (PubMed: 26401034) »
  • Shih HH  et al. Hepatitis B surface antigen levels and sequences of natural hepatitis B virus variants influence the assembly and secretion of hepatitis d virus. J Virol 82:2250-64 (2008). WB . Read more (PubMed: 18094179) »

See all 2 Publications for this product

Product Wall

Anti-Human IgG antibody ab6858 has not been tested for reactivity with other species. For an antibody that is exclusively reactive with human IgG, we recommend ab97175.

Thank you for contacting us. For this secondary antibody we recommend a 1/180K dilution with ELISA. However, you will need to optimise and determine the best dilutions for your specific assay; considering starting dilutions from 1/40000. I hope this in...

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Thank you for contacting us. Pre-adsorbed antibodies are more specific towards primary antibodies than the non pre-adsorbed products. Usually, a conjugated antibody is purified by affinity chromatography using an antigen of interest coupled to ...

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Thank you for your recent telephone enquiry. I can confirm that these two antibodies are essentially the same. The difference is that ab7153 is further purified by preadsorbtion to minimize its cross reactivity to 10 serum other serum proteins. ...

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