Goat Anti-Human IgG H&L (HRP) (ab81202)

Overview

  • Product nameGoat Anti-Human IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityNo crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    Human IgG.

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.50
    Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesFrom the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100.
ELISA 1/1000.

References for Goat Anti-Human IgG H&L (HRP) (ab81202)

ab81202 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your questions. Ab81202 was raised against human IgG, and since ab81202 is polyclonal, I expect it to react with both the heavy and light chains. I do not think that this has been specifically tested. Ab81202 has...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"