Goat Anti-Human IgG H&L (HRP) (ab81202)

Overview

  • Product name
    Goat Anti-Human IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target species
    Human
  • Specificity
    No crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Human IgG.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.50
    Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound.
  • Conjugation notes
    The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100.
ELISA 1/1000.

References for Goat Anti-Human IgG H&L (HRP) (ab81202)

ab81202 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your questions. Ab81202 was raised against human IgG, and since ab81202 is polyclonal, I expect it to react with both the heavy and light chains. I do not think that this has been specifically tested. Ab81202 has...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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