Goat Anti-Human IgG H&L (HRP) preadsorbed (ab97175)
Key features and details
- Goat Anti-Human IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
-
Product name
Goat Anti-Human IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
-
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat more details -
Immunogen
Other Immunogen Type corresponding to Human IgG.
-
Conjugation
HRP
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use at an assay dependent dilution.
|
|
IHC-P |
1/200 - 1/5000.
|
|
ELISA |
1/10000 - 1/100000. ELISA-Primary
|
|
WB |
1/5000 - 1/50000. WB-Colorimetric 1/5000 - 1/30000; WB-Chemiluminescent 1/10000 - 1/50000
|
Notes |
---|
ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/5000. |
ELISA
1/10000 - 1/100000. ELISA-Primary |
WB
1/5000 - 1/50000. WB-Colorimetric 1/5000 - 1/30000; WB-Chemiluminescent 1/10000 - 1/50000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (11)
ab97175 has been referenced in 11 publications.
- Lenhart-Pendergrass PM et al. Deficient Complement Opsonization Impairs Mycobacterium avium Killing by Neutrophils in Cystic Fibrosis. Microbiol Spectr 11:e0327922 (2023). PubMed: 36651756
- Rafat N et al. Enhanced Enzymatically Amplified Metallization on Nanostructured Surfaces for Multiplexed Point-of-Care Electrical Detection of COVID-19 Biomarkers. Small 18:e2203309 (2022). PubMed: 36036173
- Nick JA et al. Host and pathogen response to bacteriophage engineered against Mycobacterium abscessus lung infection. Cell 185:1860-1874.e12 (2022). PubMed: 35568033
- Malcolm KC et al. Specificity of Immunoglobulin Response to Nontuberculous Mycobacteria Infection in People with Cystic Fibrosis. Microbiol Spectr 10:e0187422 (2022). PubMed: 35863022
- Harpaz D et al. Enhanced Colorimetric Signal for Accurate Signal Detection in Paper-Based Biosensors. Diagnostics (Basel) 10:N/A (2020). PubMed: 31936174