Goat Anti-Human IgG H&L (HRP) preadsorbed (ab97175)

Overview

  • Product name
    Goat Anti-Human IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Human IgG - H&L (HRP), pre-adsorbed
  • Target species
    Human
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Human IgG.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. ELISA-Primary
WB 1/5000 - 1/50000. WB-Colorimetric 1/5000 - 1/30000; WB-Chemiluminescent 1/10000 - 1/50000

References for Goat Anti-Human IgG H&L (HRP) preadsorbed (ab97175)

ab97175 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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