Goat polyclonal Secondary Antibody to Human IgG - H&L (HRP), pre-adsorbed
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
Purification notesAntiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Conjugation notesMolar enzyme/ antibody protein ratio is 4:1