Goat Anti-Human IgG H&L preadsorbed (ab97166)
Key features and details
- Goat Anti-Human IgG H&L preadsorbed
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Human IgG H&L preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, mouse, pig, rabbit and rat was detected.
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Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Chicken, Cow, Horse, Mouse, Pig, Rabbit, Rat more details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/100 - 1/10000. ELISA-Coating 1/100 - 1/500; ELISA-Primary 1/1000 - 1/10000
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WB |
1/1000 - 1/30000. WB-Colorimetric 1/1000 - 1/10000; WB-Chemiluminescent 1/1000 - 1/30000
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/10000. ELISA-Coating 1/100 - 1/500; ELISA-Primary 1/1000 - 1/10000 |
WB
1/1000 - 1/30000. WB-Colorimetric 1/1000 - 1/10000; WB-Chemiluminescent 1/1000 - 1/30000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab97166 has been referenced in 2 publications.
- Boysen L et al. Generic immune complex assay for detection of murine anti-drug-antibodies in complex with human IgG. Biologicals 60:42-48 (2019). PubMed: 31200984
- Schröder C et al. Late-Onset Antibody Deficiency Due to Monoallelic Alterations in NFKB1. Front Immunol 10:2618 (2019). PubMed: 31803180