Goat Anti-Human IgM mu chain (Agarose) preadsorbed (ab65868)

Overview

  • Product nameGoat Anti-Human IgM mu chain (Agarose) preadsorbed
    See all IgM secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Human IgM - mu chain (Agarose), pre-adsorbed
  • Target speciesHuman
  • Specificityµ-chain specific. This antibody is specific for human IgM when tested against purified human IgA, IgG, IgM, and kappa and lambda light chains.
  • Tested applicationsSuitable for: Purificationmore details
  • Minimal
    cross-reactivity

    Mouse, Rat more details
  • ConjugationAgarose

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Agarose beads suspension (1:1) in 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purification notesAffinity isolated antibody is obtained from goat anti-human IgM antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the µ-chain of human IgM. The antibody preparation is solid phase adsorbed with mouse and rat IgG's to ensure minimal cross-reactivity in tissue or cell preparations. Affinity isolated antibody is coupled to cyanogen-bromide activated agarose at 3-4 mg antibody per milliliter of resin.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes


    Because of the minimal interspecies cross reactivity to mouse or rat IgG's this product is excellent for screening human monoclonal antibodies produced by hybridoma cells grown in systems containing mouse or rat immunoglobulins. It has the ability to detect and separate human IgM in human biological fluids or tissues in normal or pathological situations such as cancer or autoimmune diseases.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent dilution. 1ml of Antibody-Agarose suspension binds 2-3 mg human IgM.

References for Goat Anti-Human IgM mu chain (Agarose) preadsorbed (ab65868)

ab65868 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"