Purification notesAnti-Human IgM (µ-chain specific) is prepared from
goat anti-human IgM antiserum by immunospecific
purification to remove essentially all goat serum protein,
including immunoglobulins, which do not bind
specifically to the µ-chain of human IgM.
Conjugation notesConjugation of the antibody preparation to Alkaline Phosphatase is accomplished by protein cross-linking with 0.2% glutaraldehyde.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: A minimum titer of 1:7,000 is determined by Direct ELISA. Titer is defined as the dilution of conjugate sufficient to give a change in absorbance of 1.0 at 405 nm after 30 minutes of substrate conversion at 25 °C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Goat Anti-Human IgM mu chain (Alkaline Phosphatase) (ab49631)
has not yet been referenced specifically in any publications.
Publishing research using ab49631? Please let us know so that we can cite the reference in this datasheet.
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