Goat Anti-Human IgM mu chain (Alkaline Phosphatase) (ab49631)

Overview

  • Product name
    Goat Anti-Human IgM mu chain (Alkaline Phosphatase)
    See all IgM secondary antibodies
  • Target species
    Human
  • Specificity
    The conjugate is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones Kappa, and Lambda myeloma proteins.
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    -

  • Epitope
    µ-chain specific
  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.05M Tris buffered saline, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Anti-Human IgM (µ-chain specific) is prepared from goat anti-human IgM antiserum by immunospecific purification to remove essentially all goat serum protein, including immunoglobulins, which do not bind specifically to the µ-chain of human IgM.
  • Conjugation notes
    Conjugation of the antibody preparation to Alkaline Phosphatase is accomplished by protein cross-linking with 0.2% glutaraldehyde.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: A minimum titer of 1:7,000 is determined by Direct ELISA. Titer is defined as the dilution of conjugate sufficient to give a change in absorbance of 1.0 at 405 nm after 30 minutes of substrate conversion at 25 °C.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab49631 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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