Goat Anti-Human IgM mu chain (HRP) preadsorbed (ab49690)

Overview

  • Product nameGoat Anti-Human IgM mu chain (HRP) preadsorbed
    See all IgM secondary antibodies
  • Target speciesHuman
  • SpecificityThis antibody reacts with the mu chain of human IgM. It does not react with other human Igs. It is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones kappa and lambda myeloma proteins. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.
  • Tested applicationsSuitable for: ELISA, IHC-P, Dot blotmore details
  • Minimal
    cross-reactivity

    Mouse, Rat more details
  • Immunogen

    Full length protein, Human IgM.

  • ConjugationHRP

Properties

Applications

Our Abpromise guarantee covers the use of ab49690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
Dot blot
  • Application notesDot: A minimum dilution of 1:6,000 was determined by direct dot blot assay using 40 ng human IgM/dot. A minimum dilution of 1:100,000 was determined in a direct chemiluminescence assay using 20 ng human IgM/dot. Luminol plus enhancer was used as substrate.

    ELISA (direct): 1/50000.
    IHC-P: 1/200.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Human IgM mu chain (HRP) preadsorbed (ab49690)

    ab49690 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"