Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride, 0.1% BSA, 50% Glycerol
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Isotype
    IgG
  • General notes

    Number of blots:
    At least 20 blots (based on a 1 µl/ml dilution in 5 ml milk).

    Important protocol notes:

    1. The anti-goat VeriBlot for IP secondary antibody (HRP) detects goat IgG antibodies.

    2. The anti-goat VeriBlot for IP secondary antibody (HRP) preferentially detects the non-reduced form of goat IgG over the reduced, SDS-denatured forms.

    3. IP sample should be completely reduced/denatured before loaded onto a western blot.

    4. Milk should be used as the blocking protein for the immunoblot.

    Western blot and IP resources:
    a) Western blot a beginner's guide
    b) IP protocol
    c) IP troubleshooting tips

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. The dilution will depend on the sensitivity of the HRP substrate. The recommended dilution is 1 µl in 1 ml milk. Based on the recommended dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples.

References

This product has been referenced in:
  • Grimaldo L  et al. Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels. PLoS One 12:e0185289 (2017). Read more (PubMed: 28957379) »
  • Feng G  et al. microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway. Mol Med Rep 14:1631-5 (2016). WB . Read more (PubMed: 27314868) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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