Goat IgG was coupled to agarose beads using a cyanogen bromide method.
Goat IgG concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0mg of IgG, before coupling to agarose beads.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Use 15-25µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.
ab104155 is an Immunoprecipitation (IP) control that is used in conjuction with agarose-immobilize antibodies made in goat.