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Product Name
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Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP)
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Product type
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Secondary antibodies
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Description
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Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP)
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Immunogen
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Full length native Goat IgG (purified).
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Tested applications
(see key)
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Dot, ELISA, IHC-Fr, IHC-P, IM, WB
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependant dilution.
ELISA: 1/2000 - 1/8000.
IHC-P: Use at an assay dependant dilution.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Research areas
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Secondary antibodies >> anti-Goat >> IgG >> Enzyme >> Alkaline Phos
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
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Alternative names Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab6886) |
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Raised in
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Donkey
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, 0.05% Tris HCl, 10mg/ml BSA, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride. pH 8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
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Form
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Liquid
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Conjugation
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Alkaline Phosphatase
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to IgG
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