Goat IgG secondary antibody - H&L - F(ab)2 Fragment (ab7004)

Product Name 

Goat IgG secondary antibody - H&L - F(ab)2 Fragment

Product type 

Secondary antibodies

Description 

Donkey polyclonal to Goat IgG - H&L - F(ab)2 Fragment (PE)

Immunogen 

Goat IgG whole molecule

Specificity 

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, or Rat
Serum Proteins or other Goat heavy or light chain proteins.

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IHC-P, IM

Application notes 
(see key)

Recommended dilutions
The maximum amount of reagent required to stain 1 x 10⁶ cells in flow cytometry is approximately 1.0µg of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.
As a general guideline, dilutions of 1:50 to 1:200 should be suitable for most applications.

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Goat >> IgG >> Fluorophore >> Phycoerythrin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Raised in 

Donkey

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Conjugation notes 

R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons)
Absorption Wavelength: 488 nm
Emission Wavelength: 575 nm
OD_566nm/OD_280nm: 2.8

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Purification notes 

Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

Form 

Liquid

Conjugation 

Phycoerythrin

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This antibody is a F(ab')₂ fragment.

References for Goat IgG secondary antibody - H&L - F(ab)2 Fragment (ab7004)

  This product has been used in:

Miles A et al. CCL25 and CCL28 promote alpha4 beta7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow. Am J Physiol Gastrointest Liver Physiol 294:G1257-67 (2008). PubMed: 18308860

If you publish research using ab7004 please let us know so that we can cite the reference on this datasheet.

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