Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed (ab50596)

Overview

  • Product nameGoat Anti-Mouse IgG F(ab) (TRITC) preadsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityThe conjugate can detect mouse IgA, mouse IgM, and all subclasses of mouse IgG. The conjugate shows no reactivity with mouse IgG Fc fragment.
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Purified mouse IgG, Fab fragment

  • Positive controlHuman peripheral blood lymphocytes and human tonsil sections
  • ConjugationTRITC. Ex: 547nm, Em: 572nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.
  • Conjugation notesAb50596 is conjugated to crystalline tetramethylrhodamine isothiocyantate (TRITC) in an alkaline reaction, then further purified to remove unbound TRITC.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF 1/32.

Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed images

  • Immunofluorescent staining of microglia in the hippocampus (CA3 region) in rat brain. Rat brain sections stained with Monoclonal Anti-Glial Fibrillary Acidic Protein (clone no. G-A-5), and ab50596 at 1/32 dilution.

References for Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed (ab50596)

ab50596 has not yet been referenced specifically in any publications.

Product Wall

Thank you for tour reply.

The Goat polyclonal Secondary Antibody to Mouse IgG - Fab' (TRITC ), pre-adsorbed can now be viewed from the datasheet under the catalogue number ab50596.

http://www.abcam.com/ab50596

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Thank you for contacting us and I am very sorry for the late reply to your enquiry.

I am pleased to say that we now do have the Goat polyclonal Secondary Antibody to Mouse IgG - Fab' (TRITC ), pre-adsorbed (ab50596) available in it's unconjug...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"