Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed (ab50596)

Overview

  • Product name
    Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Specificity
    The conjugate can detect mouse IgA, mouse IgM, and all subclasses of mouse IgG. The conjugate shows no reactivity with mouse IgG Fc fragment.
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Purified mouse IgG, Fab fragment

  • Positive control
    Human peripheral blood lymphocytes and human tonsil sections
  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.
  • Conjugation notes
    Ab50596 is conjugated to crystalline tetramethylrhodamine isothiocyantate (TRITC) in an alkaline reaction, then further purified to remove unbound TRITC.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF 1/32.

Images

  • Immunofluorescent staining of microglia in the hippocampus (CA3 region) in rat brain. Rat brain sections stained with Monoclonal Anti-Glial Fibrillary Acidic Protein (clone no. G-A-5), and ab50596 at 1/32 dilution.

References

This product has been referenced in:
  • Ho M  et al. Recombinant botulinum neurotoxin A heavy chain-based delivery vehicles for neuronal cell targeting. Protein Eng Des Sel 24:247-53 (2011). Read more (PubMed: 21051321) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for tour reply.

The Goat polyclonal Secondary Antibody to Mouse IgG - Fab' (TRITC ), pre-adsorbed can now be viewed from the datasheet under the catalogue number ab50596.

http://www.abcam.com/ab50596

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Thank you for contacting us and I am very sorry for the late reply to your enquiry.

I am pleased to say that we now do have the Goat polyclonal Secondary Antibody to Mouse IgG - Fab' (TRITC ), pre-adsorbed (ab50596) available in it's unconjug...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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