Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed (ab50596)

Overview

  • Product name
    Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Specificity
    The conjugate can detect mouse IgA, mouse IgM, and all subclasses of mouse IgG. The conjugate shows no reactivity with mouse IgG Fc fragment.
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Purified mouse IgG, Fab fragment

  • Positive control
    Human peripheral blood lymphocytes and human tonsil sections
  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.
  • Conjugation notes
    Ab50596 is conjugated to crystalline tetramethylrhodamine isothiocyantate (TRITC) in an alkaline reaction, then further purified to remove unbound TRITC.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF 1/32.

Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed images

  • Immunofluorescent staining of microglia in the hippocampus (CA3 region) in rat brain. Rat brain sections stained with Monoclonal Anti-Glial Fibrillary Acidic Protein (clone no. G-A-5), and ab50596 at 1/32 dilution.

References for Goat Anti-Mouse IgG F(ab) (TRITC) preadsorbed (ab50596)

ab50596 has not yet been referenced specifically in any publications.

Product Wall

Thank you for tour reply.

The Goat polyclonal Secondary Antibody to Mouse IgG - Fab' (TRITC ), pre-adsorbed can now be viewed from the datasheet under the catalogue number ab50596.

http://www.abcam.com/ab50596

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Thank you for contacting us and I am very sorry for the late reply to your enquiry.

I am pleased to say that we now do have the Goat polyclonal Secondary Antibody to Mouse IgG - Fab' (TRITC ), pre-adsorbed (ab50596) available in it's unconjug...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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