Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49678)

Overview

  • Product name
    Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Specificity
    The conjugate is specific for IgG and IgG, Fc fragment. The antibody reacts with subclasses IgG1, IgG2a, IgG2b and IgG3, but not with IgA or IgM.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Dot blotmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Purified IgG, Fc fragment (Mouse)

  • Positive control
    Human tonsil
  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.05M Tris, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody preparation is solid phase adsorbed with Human IgG to ensure minimal cross reactivity in tissue or cell preparations.
  • Conjugation notes
    Conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Predicted molecular weight: 40 kDa.
ELISA 1/40000.
IHC-P 1/25.
Dot blot 1/20000 - 1/50000.

References

ab49678 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please check the following datasheets as answer to your first question;

http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

http://www.abcam.com/Go...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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