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Product name Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed See all IgG secondary antibodies
Target species Mouse
Specificity Binds IgG; does not bind other Igs.
Tested applications Suitable for:
WB, ELISA, IHC-P, Dot blot more details
Minimal cross-reactivity Cow, Horse, Human
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Purified mouse IgG, Fc fragment
Positive control Human tonsil tissue
Conjugation Alkaline Phosphatase Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 8.00 Preservative: 0.25% Sodium azide Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA
Concentration information loading...
Purity Immunogen affinity purified
Purification notes The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Solid phase adsorption with bovine and horse serum
proteins ensures minimal cross reactivity with horse or fetal calf serum in hybridoma media
Conjugation notes Goat anti-mouse IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Dot: 1/30000 - 1/100000.
WB: 1/160000 when using chemiluminescent detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49724)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"