Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49724)

Overview

  • Product nameGoat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityBinds IgG; does not bind other Igs.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Dot blotmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Purified mouse IgG, Fc fragment

  • Positive controlHuman tonsil tissue
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8.00
    Preservative: 0.25% Sodium azide
    Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Solid phase adsorption with bovine and horse serum proteins ensures minimal cross reactivity with horse or fetal calf serum in hybridoma media
  • Conjugation notesGoat anti-mouse IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
Dot blot
  • Application notesDot: 1/30000 - 1/100000.
    ELISA: 1/30000.
    IHC-P: 1/40.
    WB: 1/160000 when using chemiluminescent detection.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49724)

    ab49724 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"