Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49724)

Overview

  • Product name
    Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Specificity
    Binds IgG; does not bind other Igs.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Dot blotmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Purified mouse IgG, Fc fragment

  • Positive control
    Human tonsil tissue
  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.25% Sodium azide
    Constituents: 0.06% Tris buffered saline, 0.0095% Magnesium chloride, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Solid phase adsorption with bovine and horse serum proteins ensures minimal cross reactivity with horse or fetal calf serum in hybridoma media
  • Conjugation notes
    Goat anti-mouse IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
Dot blot
  • Application notes
    Dot: 1/30000 - 1/100000.
    ELISA: 1/30000.
    IHC-P: 1/40.
    WB: 1/160000 when using chemiluminescent detection.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Mouse IgG Fc (Alkaline Phosphatase) preadsorbed (ab49724)

    ab49724 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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