Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
1/50 - 1/500.
1/50 - 1/200.
Goat Anti-Mouse IgG Fc (DyLight® 550) images
DyLight® - Goat polyclonal Secondary Antibody to Mouse IgG - Fc (DyLight® 550) (ab97016)
Emission spectra of DyLight® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
References for Goat Anti-Mouse IgG Fc (DyLight® 550) (ab97016)
has not yet been referenced specifically in any publications.
Publishing research using ab97016? Please let us know so that we can cite the reference in this datasheet.
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