Overview

  • Product nameGoat Anti-Mouse IgG Fc (FITC)
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applicationsIHC-P, ICC/IF, Flow Cytmore details
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notesF/P ratio is 4.4
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Mouse IgG Fc (FITC) images

  • A431 cell nucleus (DAPI signal) showing three gamma-H2AX foci structures.
    Primary anitibody H2A.X (phospho S139) antibody [3F2], ab22551, 100ug.
    Secondary antibody Mouse IgG-Fc (FITC), ab97264, 1mg.

References for Goat Anti-Mouse IgG Fc (FITC) (ab97264)

This product has been referenced in:
  • González JE  et al. Radiosensitization induced by the anti-epidermal growth factor receptor monoclonal antibodies cetuximab and nimotuzumab in A431 cells. Cancer Biol Ther 13:71-6 (2012). ICC/IF ; Human . Read more (PubMed: 22231391) »

See 1 Publication for this product

Product Wall

Thanks for sending the purchase order information. I have located the order for ab97264. Would you like me to send another vial of this same product as the replacement?

Thanks for the reply. So I can certainly set up the replacement to send one vial of ab6724. For some reason the purchase order information did not make it through. If you could, please re-send this and I'll be happy to set up the replacement ord...

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Since the antibody is not working as expected I would be happy to send a replacement. I just need a bit more information to process your request: 1. What antibody would you like as the replacement? 2. What is the order number used to purchase...

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You are correct that if we find the antibody is not peforming as expected, based on information on our online datasheet, we would be happy to offer a replacement, credit or refund. In this case, the antibody may be reacting unspecifically. Based...

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Thank you for your enquiry. I would recommend that the customer increase the dilition factor for this secondary antibody from 1:50 to 1:200. I believe this will reduce the background. I hope this is helpful. Please let me know if there a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"