Overview

  • Product nameGoat Anti-Mouse IgG Fc (HRP)
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityBased on immunoelectrophoresis, reacts with the heavy chains on mouse IgG but not with the light chains on most mouse immunoglobulins. No antibody was detected against mouse IgM, or against non-immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.
  • Tested applicationsSuitable for: IHC, ELISA, WBmore details
  • Immunogen

    Mouse IgG Fc fragment.

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: None
    Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate. pH 7.6
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesIsolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
  • Conjugation notesUse of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC 1/500 - 1/5000.
ELISA 1/5000 - 1/100000.
WB 1/10000 - 1/200000. Predicted molecular weight: 40 kDa.

Goat Anti-Mouse IgG Fc (HRP) images



  • Predicted band size : 40 kDa
    Ab20043 was used at dilution 1/3000 with the primary antibody ab487 in WB. See the review on ab487.

References for Goat Anti-Mouse IgG Fc (HRP) (ab20043)

This product has been referenced in:
  • Xiao S  et al. Genome-scale RNA interference screen identifies antizyme 1 (OAZ1) as a target for improvement of recombinant protein production in mammalian cells. Biotechnol Bioeng 113:2403-15 (2016). Read more (PubMed: 27215166) »
  • Meier D  et al. Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes. PLoS One 10:e0137229 (2015). WB . Read more (PubMed: 26361389) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

Please check the following datasheets as answer to your first question;

http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

http://www.abcam.com/Go...

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Thank you for contacting us.
I am sorry to hear you are having problems with one of our products. I appreciate the details provided. I am afraid that product ab41616 is not guaranteed to work in ELISA. We have tested this antibody in WB and ICC, b...

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Thank you for your reply. We do not provide samples of our antibodies but under our Abpromise we do guarantee our products to work as stated on the datasheet. If they fail to perform as stated then we will happily replace or refund the antibody...

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Thank you for contacting Abcam. Based on your requirements, I would recommend ab20043 as it will only react with the heavy chain of the mouse IgG. The image below shows the different regions of the IgG molecule and so from that you will be able to see ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"