• Product nameGoat Anti-Mouse IgG Fc (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP)
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
  • ConjugationHRP


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: None
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab97265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000

Goat Anti-Mouse IgG Fc (HRP) images

  • IHC image of beta actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97265,  1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/200 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References for Goat Anti-Mouse IgG Fc (HRP) (ab97265)

This product has been referenced in:
  • Huang ZJ  et al. Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells. Mol Med Rep 11:1647-54 (2015). WB . Read more (PubMed: 25405855) »
  • Lee JK  et al. Age and the means of bypassing stasis influence the intrinsic subtype of immortalized human mammary epithelial cells. Front Cell Dev Biol 3:13 (2015). WB ; Human . Read more (PubMed: 25815289) »

See all 8 Publications for this product

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This antibody should work for all IgG subclasses. Although you know your primary works, perhaps it is somewhat less efficient than the primary antibody with the IgG2a subclass?

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Yes, this antibody will react with the Fc domain of all mouse IgG including IgG1, but not IgA or IgM.

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Please find attached certificate of compliance. We do not provide certificate of analysis for antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mor...

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Merci de nous avoir contactés.
Veuillez trouver ci-joint le certificat dont vous avez fait la demande.
J'espère que ce document vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Wenn Sie in Zukunft ein Produkt testen möchten, oder allgemin mehr über einen Antikörper oder eines unserer Produkte wissen möchten, wenden Sie sich an uns vom technischen Dienst (wissenschaftlicherdienst@abcam.com). Frau Langenick arbeitet i...

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We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

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The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the...

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Thank you for contacting us.  I am still waiting for additional information regarding the strain used to generate ab106292, but I will let you know as soon as I hear back from the lab regarding this.  The other products are as follows: ab34749: 9B11 wa...

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Thank you for contacting us. Unfortunately I am not able to provide information about the exact antibody binding sequence for ab97265 as this is considered proprietary by the lab that generated the product. As stated on the product specificity, ...

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