Overview

  • Product nameGoat Anti-Mouse IgG Fc (HRP)See all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP)
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applicationsICC, IHC-P, ELISA, WB more details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/25000. Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000

References for Goat Anti-Mouse IgG Fc (HRP) (ab97265)

This product has been referenced in:
  • Peng CW  et al. Combined features based on MT1-MMP expression, CD11b + immunocytes density and LNR predict clinical outcomes of gastric cancer. J Transl Med 11:153 (2013). Read more (PubMed: 23787075) »
  • Brumbaugh AR  et al. Immunization with the yersiniabactin receptor, FyuA, protects against pyelonephritis in a murine model of urinary tract infection. Infect Immun 81:3309-16 (2013). Read more (PubMed: 23798537) »

See all 4 Publications for this product

Product Wall

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This antibody should work for all IgG subclasses. Although you know your primary works, perhaps it is somewhat less efficient than the primary antibody with the IgG2a subclass?

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Yes, this antibody will react with the Fc domain of all mouse IgG including IgG1, but not IgA or IgM.

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Please find attached certificate of compliance. We do not provide certificate of analysis for antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any mor...

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Merci de nous avoir contactés.
Veuillez trouver ci-joint le certificat dont vous avez fait la demande.
J'espère que ce document vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.



Wenn Sie in Zukunft ein Produkt testen möchten, oder allgemin mehr über einen Antikörper oder eines unserer Produkte wissen möchten, wenden Sie sich an uns vom technischen Dienst (wissenschaftlicherdienst@abcam.com). Frau Langenick arbeitet i...

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We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

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The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the...

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Thank you for contacting us.  I am still waiting for additional information regarding the strain used to generate ab106292, but I will let you know as soon as I hear back from the lab regarding this.  The other products are as follows: ab34749: 9B11 wa...

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Thank you for contacting us. Unfortunately I am not able to provide information about the exact antibody binding sequence for ab97265 as this is considered proprietary by the lab that generated the product. As stated on the product specificity, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"