Goat Anti-Mouse IgG H&L (6nm Gold) (ab39614)

Overview

  • Product nameGoat Anti-Mouse IgG H&L (6nm Gold)
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: Electron Microscopymore details
  • Immunogen

    Mouse IgG whole molecule.

  • ConjugationGold 6nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 6nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 12 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notesElectron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 6nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also van Bergen en Henegouwen & Leunissen, Histochemistry, 85, (1986), 81.)

    Light Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Mouse IgG H&L (6nm Gold) (ab39614)

    ab39614 has not yet been referenced specifically in any publications.

    Product Wall

    I was informed that the 6nm gold particles will be visible in FESEM, however intensity of the signal (brightness of the white spots) will be low. A Goat polyclonal Secondary Antibody to Mouse IgG - H&L (10nm Gold) (ab39619) might be more appropriate. ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"