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Our Abpromise guarantee covers the use of ab97020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|ELISA||1/5000 - 1/50000. (Primary ELISA)|
|WB||1/5000 - 1/50000. (Chemiluminescent)|
|IHC-P||1/100 - 1/1000.|
All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
Lanes 1 -6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/20000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes