Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC, ELISA, WB, IHC-Pmore details
  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notes
    Molar enzyme: antibody protein ratio is 1:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
ELISA 1/5000 - 1/50000. (Primary ELISA)
WB 1/5000 - 1/50000. (Chemiluminescent)
IHC-P 1/100 - 1/1000.

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) images

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1 -6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/5000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/20000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/50000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)

This product has been referenced in:
  • Haghani A  et al. Edible bird's nest modulate intracellular molecular pathways of influenza A virus infected cells. BMC Complement Altern Med 17:22 (2017). Read more (PubMed: 28056926) »
  • Mehrbod P  et al. Simvastatin modulates cellular components in influenza A virus-infected cells. Int J Mol Med 34:61-73 (2014). WB . Read more (PubMed: 24788303) »

See all 3 Publications for this product

Product Wall

Tubulin

Excellent
Abreviews
Application
Western blot
5 ug of cell spermatocyte lysate with RIPA buffer
30 min skim milk blocking
Primary 1:2000 room temp for 1 hr
Secondary 1:2000 Room temp

Detection with Alkaline phosphatase substrate
Username

Abcam user community

Verified customer

Submitted Jul 06 2016

Thank you for contacting us.

Please check the catalogue number ab5000; http://www.abcam.com/6X-His-tag-antibody-4D11-ab5000.html

ab97020; http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-HL-AP-ab97020.html

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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