AbExcel

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)

Overview

  • Product nameGoat Anti-Mouse IgG H&L (Alkaline Phosphatase)See all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP)
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsICC, ELISA, WB, IHC-P more details
  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notesMolar enzyme: antibody protein ratio is 1:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use at an assay dependent dilution.
ELISA 1/5000 - 1/50000. (Primary ELISA)
WB 1/5000 - 1/50000. (Chemiluminescent)
IHC-P 1/100 - 1/1000.

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) images

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1 -6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/5000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/20000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AP) (ab97020) at 1/50000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)

This product has been referenced in:

See 1 Publication for this product

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Thank you for contacting us.

Please check the catalogue number ab5000; http://www.abcam.com/6X-His-tag-antibody-4D11-ab5000.html

ab97020; http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-HL-AP-ab97020.html

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"