Goat Anti-Mouse IgG H&L (Chromeo™ 546) (ab60316)

Overview

  • Product nameGoat Anti-Mouse IgG H&L (Chromeo™ 546)See all IgG secondary antibodies ...
  • Target speciesMouse
  • Specificityab60316 reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applicationsICC/IFmore details
  • ConjugationChromeo™ 546

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Conjugation notesChromeo™ 546 is spectrally similar to Cy3®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 545 and 568 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 96,800 M-1cm-1 (measured at Amax) Quantum Yield: ~ 11 % Excitation Wavelength Range: 530 to 550 nm Emission Wavelength Range: 560 to 570 nm
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab60316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/2000.
Plate-based assays: 1/800 to 1/1000.

Goat Anti-Mouse IgG H&L (Chromeo™ 546) images

  • EGF receptor detected in an urothel cell line by ICC/IF using ab60316 at 1/1000. Paraffin-treated tissue.
  • ab60316 staining a mouse monoclonal antibody to alpha Tubulin in HeLa cells by Immunocytochemistry/Immunofluorescense.

References for Goat Anti-Mouse IgG H&L (Chromeo™ 546) (ab60316)

ab60316 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Rat primary neuronal cultures where grown on cover-slips. The cells where fixed using 4% PFA. Post fixation the cells where permeabilized with 0.25% Triton-X and blocking was performed with PBS containing 3% bovine serum albumin and 5% goat serum for 1 h at 25C.

The cultures were stained with GR specific primary antibody BuGR2(1 : 200, Abcam Ab) for 1 h. After multiple washes with PBS Cultures were stained with incubation with 1 : 1000 dilution of the secondary anti-mouse IgG antibody ChromeoTM 546 (Abcam) for 1 h at 25C. The cells were finally washed with PBST (PBS with 0.01% Triton-X) and mounted after staining with DAPI nuclear stain.
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Submitted Dec 09 2013

Application Immunocytochemistry/ Immunofluorescence
Rat primary neuronal cultures where grown on cover-slips. The cells where fixed using 4% PFA. Post fixation the cells where permeabilized with 0.25% Triton-X and blocking was performed with PBS containing 3% bovine serum albumin and 5% goat serum for 1 h at 25C.

The cultures were stained with GR specific primary antibody BuGR2(1 : 200, Abcam Ab) for 1 h. After multiple washes with PBS Cultures were stained with incubation with 1 : 1000 dilution of the secondary anti-mouse IgG antibody ChromeoTM 546 (Abcam) for 1 h at 25C. The cells were finally washed with PBST (PBS with 0.01% Triton-X) and mounted after staining with DAPI nuclear stain.
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Verified customer

Submitted Nov 26 2013

Thank you for contacting us yesterday.
As discussed over the phone, I have looked into whether there might be any suitable alternative to the Goat anti-Mouse IgG H&L (Chromeo™ 546) secondary antibody (ab60316) for you. Particularly in regards to s...

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Thank you for contacting us.
I am very happy to help you find the most suitable antibodies for your application. For that, could you please specify if you are interested in performing immunostaining in tissues or immunocytochemistry?
Please n...

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Thank you for contacting us.

Yes, those two antibodies, ab5714 and ab60316, are a compatible pair, and ab5714 will recognize rat Bax.

Please note that ab5714 should not be stored frozen, but only at 4oC.

I hope this inf...

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When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"