Goat Anti-Mouse IgG H&L (Chromeo™ 642) (ab60318)

Overview

  • Product nameGoat Anti-Mouse IgG H&L (Chromeo™ 642)
    See all IgG secondary antibodies
  • Target speciesMouse
  • Specificityab60318 reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applicationsICC/IFmore details
  • Immunogen

    Mouse IgG.

  • ConjugationChromeo™ 642

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Conjugation notesChromeo™ 642 is spectrally similar to Cy5®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 642 and 666 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 180,000 M-1cm-1 (measured at Amax) Quantum Yield: ~ 7 % Excitation Wavelength Range: 630 to 655 nm Emission Wavelength Range: 650 to 670 nm
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab60318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notesICC/IF: 1/1000 - 1/2000.
    Plate-based assays: 1/800 to 1/1000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat Anti-Mouse IgG H&L (Chromeo™ 642) images

    • ab60318 staining a mouse monoclonal antibody to alpha Tubulin in HeLa cells by Immunocytochemistry/Immunofluorescense with a DAPI nuclear counterstain.

    References for Goat Anti-Mouse IgG H&L (Chromeo™ 642) (ab60318)

    ab60318 has not yet been referenced specifically in any publications.

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    When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG-...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"