Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Goat
- Suitable for: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
Immunogen
Full length native protein (purified) corresponding to IgG.
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Conjugation
Cy2 ®. Ex: 489nm, Em: 505nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
Conjugation notes
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 5.1 moles Cy2 per mole of Goat IgG -
Clonality
Polyclonal -
General notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
1/10000 - 1/50000.
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WB |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
ELISA
1/10000 - 1/50000. |
WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Images
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Secondary
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944) at 1/1000 dilution (60 minutes at Room temperature)
Observed band size: 25, 55 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab6944 has been referenced in 11 publications.
- Saga Y et al. The cytosolic lncRNA dutA affects STATa signaling and developmental commitment in Dictyostelium. Genes Cells 28:111-128 (2023). PubMed: 36504347
- Umebayashi M et al. A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins. J Cell Biol 222:N/A (2023). PubMed: 36571579
- Beznoussenko GV et al. The Diffusion Model of Intra-Golgi Transport Has Limited Power. Int J Mol Sci 24:N/A (2023). PubMed: 36674888
- Collins C et al. Tubulin acetylation promotes penetrative capacity of cells undergoing radial intercalation. Cell Rep 36:109556 (2021). PubMed: 34407402
- Piao CS et al. Depression following traumatic brain injury in mice is associated with down-regulation of hippocampal astrocyte glutamate transporters by thrombin. J Cereb Blood Flow Metab 39:58-73 (2019). PubMed: 29135354