Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy3 ®), pre-adsorbed
  • Host species
    Goat
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat, Sheep more details
  • Conjugation
    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3®.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. PubMed: 24270184
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/2000 - 1/4000.

Images

  • ab97035 (1/200) detecting ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.

References

This product has been referenced in:
  • Paiva NM  et al. Ileal pouch of ulcerative colitis and familial adenomatous polyposis patients exhibit modulation of autophagy markers. Sci Rep 8:2619 (2018). IHC-P . Read more (PubMed: 29422639) »
  • Zeng XJ  et al. Impaired autophagic flux is associated with the severity of trauma and the role of A2AR in brain cells after traumatic brain injury. Cell Death Dis 9:252 (2018). Read more (PubMed: 29449536) »

See all 20 Publications for this product

Customer reviews and Q&As

excellent

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
We use this antibody together with the antibody ab2378 (Tryptase) in mastocytosis skin.
As working concentration we used a 1/100 dilution for 30minutes at room temperature.
Username

Abcam user community

Verified customer

Submitted Feb 14 2018

Application
Immunocytochemistry
We have used this secondary antibody for immunocytochemistry with 2,2'-thiodiethanol (TDE) mounting media. The original description of this mounting media (PMID: 17131355) reported an increase in the relative quantum yield of Cy3. Our results support this claim and the brightness seen in the red channel during image acquisition is considerable. Required exposure times/intensity settings for imaging are, consequently, very low compared to similar fluorophores.
The protocol for obtaining the image shown in the figure was a standard ICC protocol, followed by the TDE mounting procedure from the original TDE paper (PMID: 17131355), using sequential immersions in increasing concentrations of TDE. Slides were imaged on a Zeiss Observer Z1 with LED-based excitation (594 nm). Acquired images were deconvolved and the included image is a maximum intensity projection.
Username

Dr. Sam Nowitzki

Verified customer

Submitted Jul 09 2015

Thank you for your reply.
I'm pleased to hear you would like to participate in the testing discount scheme with the Goat anti-Mouse IgG H&L (Cy3 ®) secondary antibody (ab97035). The discount code is as follows:
Discount code: xxxxxxxx
Exp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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