Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)

Overview

  • Product nameGoat Anti-Mouse IgG H&L (Cy5 ®) preadsorbedSee all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy5 ®), pre-adsorbed
  • Target speciesMouse
  • Tested applicationsELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Mouse IgG, whole molecule (H&L)

  • ConjugationCy5 ®. Ex: 650nm, Em: 667nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
  • Conjugation notesCy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 12.6 moles Cy5 per mole of Goat IgG
  • Clonality Polyclonal
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species (human labelled with the fluorophore Cy5 here) in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/50000. PubMed: 18691532
ICC/IF 1/1000 - 1/5000.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed images

  • ab2792 (PDI antibody (RL90) - ER marker) staining human U2-OS (bone osteosarcoma) cells by ICC/IF. ab6563 (undiluted) was used as the secondary.

    See Abreview

References for Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)

This product has been referenced in:

See all 15 Publications for this product

Product Wall

Thank you for contacting us.

This antibody detects all mouse IgG, so it should work with both your IgG1 and IgG2 antibodies.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advi...

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Thank you for your inquiry.

I used to perform many ELISPOTs by hand as you with plastic plates. The capture antibody was coated on the plates. The PVDF membrane bottom well plates areused when using a reader to analyze the results. The membra...

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Sorry for the delay in getting back to you.

I can now confirm that the product has been cross adsorbed against the species listed as showing minimal cross reactivity. This includesBovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat,...

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Thank you for contacting us and sorry for the delay in getting back to you in regards to your query.

I have looked into the antibody you are interested in, the Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy5 ®), pre-adsorbe...

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Thank you for contacting Abcam.

All of the information regarding purification of the antibodies in our catalogue is available on our website. If we do not specify that Protein A/G was used in the purification process then they were not used t...

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Thank you for your reply.

The information for how each antibody is purified is available on our website. Just go to the webpage for each antibody and the information is there. I have attached a document that shows you where to find that infor...

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Thank you for contacting Abcam.

The antibody ab6563 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities a...

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Thank you very much for your interest in our antibodies. The difference between these two secondary antibodies it that ab6563 is pre-adsorbed against 10 species (chicken, cow, goat, guinea pig, hamster, horse, human, rabbit, rat, sheep), while ab970...

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Thank you for your phone call yesterday and for your questions about ab6563. I have been in touch with the lab, and I was told that small amounts of precipitate are common and should not affect the viability of the antibody. It is possible that the ...

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When antibody is anti-IgG (H+L) then it will react with other immunoglobulin classes (IgM, IgA, IgD, IgE) and subclasses since they all share the same light chains (either kappa or lambda). When antibody is Anti-IgG, F(ab')2 - not specific for IgG-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"