Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab97037)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Cy5 ®), pre-adsorbed
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • Conjugation
    Cy5 ®. Ex: 650nm, Em: 667nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.

References

This product has been referenced in:
  • Chaabane W & Appell ML Interconnections between apoptotic and autophagic pathways during thiopurine-induced toxicity in cancer cells: the role of reactive oxygen species. Oncotarget 7:75616-75634 (2016). Read more (PubMed: 27689330) »
  • Lu H  et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nat Cell Biol 16:1105-17 (2014). Read more (PubMed: 25266422) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your response.

ab97077 is a goat anti-rabbit secondary so it is unlikely to react with ab91493 (host species hamster). However, ab97037 is goat anti-mouse secondary antibody and it may cross-react with hamster IgG since mouse an...

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Thank you for your enquiry.

Could you please confirm if the customer would like to perform double or triple staining? I am a bit confused why the two selected secondary antibodies ie ab97037 and ab97077 (Cy5 ®) have the same fluorophore? ...

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Thank you very much for your interest in our antibodies. The difference between these two secondary antibodies it that ab6563 is pre-adsorbed against 10 species (chicken, cow, goat, guinea pig, hamster, horse, human, rabbit, rat, sheep), while ab970...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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