Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)

Overview

  • Product nameGoat Anti-Mouse IgG H&L (DyLight® 550) preadsorbedSee all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat more details
  • ConjugationDyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/50 - 1/500. DyLight® 550 replaces DyLight®549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)

This product has been referenced in:
  • Varikmaa M  et al. Role of mitochondria-cytoskeleton interactions in respiration regulation and mitochondrial organization in striated muscles. Biochim Biophys Acta 1837:232-45 (2014). Read more (PubMed: 24189374) »

See 1 Publication for this product

Product Wall

Thank you for your recent telephone enquiry. I am forwarding a link to our Dyelight information page from the Abcam website: http://www.abcam.com/index.html? pageconfig=resource&rid=12934#2 I hope this will be helpful to you. If you have any fur...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"