Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)

Overview

  • Product name
    Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594), pre-adsorbed
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • Conjugation
    DyLight® 594. Ex: 593nm, Em: 618nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • ab96881 was used at dilution 1:50 for 30 min for IHC analysis of FFPE human kidney. The primary antibody was a Mouse anti Human CD31 RTU for 30 min. High pH HIER was used as the antigen retrieval. A DAPI nuclear counterstain was also used. Right image: negative control

    This image is courtesy of Benjamin Kriederman (HistoTox Labs, Inc.)

References for Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)

This product has been referenced in:
  • Wood ER  et al. The Role of Phosphodiesterase 12 (PDE12) as a Negative Regulator of the Innate Immune Response and the Discovery of Antiviral Inhibitors. J Biol Chem 290:19681-96 (2015). Read more (PubMed: 26055709) »
  • Nguyen EB  et al. SPACA7 is a novel male germ cell-specific protein localized to the sperm acrosome that is involved in fertilization in mice. Biol Reprod 90:16 (2014). Read more (PubMed: 24307706) »

See all 3 Publications for this product

Product Wall

Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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