Overview

  • Product nameGoat Anti-Mouse IgG H&L (DyLight® 650)
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse IgG.

  • ConjugationDyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesDyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight® 650 replaces DyLight®649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
Flow Cyt 1/50 - 1/200.

Goat Anti-Mouse IgG H&L (DyLight® 650) images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Mouse IgG H&L (DyLight® 650) (ab96874)

This product has been referenced in:
  • Zhang L  et al. C-reactive protein directly suppresses Th1 cell differentiation and alleviates experimental autoimmune encephalomyelitis. J Immunol 194:5243-52 (2015). Read more (PubMed: 25917100) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"