Overview

  • Product nameGoat Anti-Mouse IgG H&L (FITC)See all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC)
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsICC/IF, IHC-P, Flow Cyt more details
  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notesF/P ratio is 5.1
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Mouse IgG H&L (FITC) images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Mouse anti-E-cadherin, 1:100
    Secondary antibody:Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022), 1:100
    Nuclei were counterstained with DAPI

References for Goat Anti-Mouse IgG H&L (FITC) (ab97022)

This product has been referenced in:
  • Li M  et al. Coxsackievirus B3-induced calpain activation facilitates the progeny virus replication via a likely mechanism related with both autophagy enhancement and apoptosis inhibition in the early phase of infection: An in vitro study in H9c2 cells. Virus Res N/A:N/A (2013). Read more (PubMed: 24177271) »
  • Grenier L  et al. The activation of DNA damage detection and repair responses in cleavage-stage rat embryos by a damaged paternal genome. Toxicol Sci 127:555-66 (2012). ICC/IF . Read more (PubMed: 22454429) »

See all 3 Publications for this product

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The safety datasheets has been published. Please download these from website.

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Unfortunately, we have not determined the pI (isoelectric) value for this product, ab205 Anti-F-actin antibody [NH3], nor any relevant goat anti-mouse IgG conjugated FITC antibodies like ab97022 and ab97039.
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Thank you for contacting us.

Unfortunately the indicated primary antibodies have not been tested with Flow Cytometry, and therefore are not covered by our Abpromise guarantee.

I would instead recommend using a different antibody aga...

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Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"