Goat Anti-Mouse IgG H&L (FITC) (ab97022)
- Product nameGoat Anti-Mouse IgG H&L (FITC)See all IgG secondary antibodies ...
- DescriptionGoat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC)
- Target speciesMouse
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Tested applicationsICC/IF, IHC-P, Flow Cyt more details
- ConjugationFITC. Ex: 493nm, Em: 528nm
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
- Conjugation notesF/P ratio is 5.1
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab97022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: 1/50 - 1/500.|
|IHC-P||IHC-P: 1/50 - 1/500.|
|Flow Cyt||Flow Cyt: 1/50 - 1/200.|
Goat Anti-Mouse IgG H&L (FITC) images
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Mouse anti-E-cadherin, 1:100
Secondary antibody:Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022), 1:100
Nuclei were counterstained with DAPI
References for Goat Anti-Mouse IgG H&L (FITC) (ab97022)
This product has been referenced in:
- Grenier L et al. The activation of DNA damage detection and repair responses in cleavage-stage rat embryos by a damaged paternal genome. Toxicol Sci 127:555-66 (2012). ICC/IF . Read more (PubMed: 22454429) »
- Murooka TT et al. HIV-infected T cells are migratory vehicles for viral dissemination. Nature 490:283-7 (2012). Read more (PubMed: 22854780) »