Overview

  • Product name
    Goat Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC)
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Conjugation notes
    F/P ratio is 5.1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Mouse IgG H&L (FITC) images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Mouse anti-E-cadherin, 1:100
    Secondary antibody:Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab97022), 1:100
    Nuclei were counterstained with DAPI

References for Goat Anti-Mouse IgG H&L (FITC) (ab97022)

This product has been referenced in:
  • Sheng Z  et al. Involvement of cancer-derived IgG in the proliferation, migration and invasion of bladder cancer cells. Oncol Lett 12:5113-5121 (2016). IF . Read more (PubMed: 28105218) »
  • Foss CA  et al. Molecular imaging of inflammation in the ApoE -/- mouse model of atherosclerosis with IodoDPA. Biochem Biophys Res Commun 461:70-5 (2015). IHC-P . Read more (PubMed: 25858322) »

See all 6 Publications for this product

Product Wall

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The safety datasheets has been published. Please download these from website.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Unfortunately, we have not determined the pI (isoelectric) value for this product, ab205 Anti-F-actin antibody [NH3], nor any relevant goat anti-mouse IgG conjugated FITC antibodies like ab97022 and ab97039.
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Thank you for contacting us.

Unfortunately the indicated primary antibodies have not been tested with Flow Cytometry, and therefore are not covered by our Abpromise guarantee.

I would instead recommend using a different antibody aga...

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Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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