Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)


  • Product name
    Goat Anti-Mouse IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Target species
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • Minimal

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native Mouse IgG (purified).

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality
  • Isotype
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab7064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 15767251
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/1000 - 1/2000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.


  • Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (FITC, pre-adsorbed) (ab7064) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Ab7064 was used at dilution 1/20 with the primary antibody ab7852 in ICC. See the review on ab7852.
  • Ab7064 was used at dilution 1/2000 with the primary antibody ab2378 in IHC-P. See the review on ab2378.


This product has been referenced in:
  • Ghezzi CE  et al. 3D Functional Corneal Stromal Tissue Equivalent Based on Corneal Stromal Stem Cells and Multi-Layered Silk Film Architecture. PLoS One 12:e0169504 (2017). Read more (PubMed: 28099503) »
  • Liu X  et al. Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2. PeerJ 5:e3642 (2017). Read more (PubMed: 28785522) »

See all 8 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Frozen sections)
This is a good antibody and a stable conjugate.
The binding is specific.
The luminous intensity of the conjugate is good and is stable (can still be evaluated after a long time).

Abcam user community

Verified customer

Submitted Oct 07 2015

Flow Cytometry
This is a good functioning antibody with a stable conjugate.
The binding is very specific and the luminous intensity of the conjugate is good.
We have used it in the flow cytometry with a 1/1000 dilution

Abcam user community

Verified customer

Submitted Oct 06 2015

Immunocytochemistry/ Immunofluorescence
Mouse Osteoblast cells were grown on cover slips for 48 hrs. Then Cells were fixed with formaldehyde vapor for 10 mins. Cells were permeabilized with Triton-X 100 for 5-7mins. 3% BSA was used for blocking (1hr). 1:100 dilution of mouse anti-talin antibody to mouse was added onto the cells and incubated for 1hr. Then 1:500 dilution of anti-mouse FITC conjugated secondary antibody was added on to the cells and incubated for 1hr. Before proceeding to each step cells were washed with PBS.

Abcam user community

Verified customer

Submitted Jul 05 2013

Thank you for confirming this details.

In my understanding this results show that the ab24590 isfaulty as it has been used with two different secondary antibodies without success. I provide you here therefore with the credit note for this ant...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hearthat the customer is notobtaining a staining with this two antibodies.

The details provided will enable us to investigate this case and will pro...

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