Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)

Overview

  • Product name Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed
  • Target speciesMouse
  • SpecificityThis antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA.
  • Tested applicationsSuitable for: In-Cell ELISA, WBmore details
  • Minimal
    cross-reactivity

    Goat, Horse, Human, Rabbit, Rat more details
  • Immunogen

    Full length protein corresponding to Mouse IgG.

  • ConjugationIRDye® 680RD

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage bufferpH: 7.4
    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
  • Concentration information loading...
  • PurityAffinity purified
  • Purification notesAffinity chromatography purified using pooled mouse IgG covalently linked to agarose.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
In-Cell ELISA 1/200 - 1/800.
WB 1/5000 - 1/25000.

 Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed images

  • All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 1/10000 dilution (Anti-GAPDH antibody)

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : Brd4 knockout HAP1 cell lysate (20 µg)
    Lane 3 : HeLa cell lysate (20 µg)

    Secondary
    Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/10000 dilution (Goat anti-Mouse IgG H&L (IRDye® 680RD))

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab128874 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773. Loading control to GAPDH ab8245 antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Merged signal (red and green). Green Anti-Brd4 antibody [EPR5150(2)]  ab128874 observed at 150 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW)-ab216773 as secondary antibody. Red - Anti-GAPDH antibody loading control, ab8245, observed at 37 kDa,

  • Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.

References for  Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)

ab216776 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"