Goat Anti-Mouse IgG - H&L (SureLight® Allophycocyanin), pre-adsorbed (ab99900)

Overview

  • Product name
    Goat Anti-Mouse IgG - H&L (SureLight® Allophycocyanin), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Tested applications
    Suitable for: Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Rabbit more details
  • Conjugation
    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm

Properties

  • Form
    Lyophilised:Reconstitute with 1 ml of ddH2O
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 10mM Tris,1 ug/ml Pentachlorophenol pH 8.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Conjugation notes
    Fluor : Protein ~1:1 Visible absorption maxima 652 Emission maximum 657
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    After product has been reconstituted, product should be stored at 2-8°C in the dark and be used within 3 months. If further dilution of the conjugate is required, use diluted material within one week.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 3 µg/ml.

Images

  • Fluorescence excitation and emission spectra of Allophycocyanin in 100mM sodium phosphate (pH7.2) + 1mM EDTA and 1mM sodium azide. The emission scan was taken with excitation at 630nm. The excitation scan was taken with an emission at 660nm. The curves were normailized to equalize peak heights.

References

ab99900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry and your interest in our products.

This secondary antibody has only been tested for Flow cytometry. However, as long as it is compatible with the primary antibody (host species, isotype), it should be fine for immun...

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Wenn Antikörper mit Biotin gekoppelt werden, wird das meist über freie Anime getan. Das bedeutet das Biotin über den ganzen Antikörper verteilt ist. Die Antikörper werden dann darauf...

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Thank you very much for your feedback.

I am happy to hear that the problem might has been resolved - please keep us updated with the results in FACS as well.

I have looked through your answers, and indeed, with that settings and all...

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Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

In order to understand the problem better and to try to find the origin of the problem, I appreciate if you could confirm the following...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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