Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

Overview

• Product name
  Goat Anti-Mouse IgG H&L (TRITC)
  See all IgG secondary antibodies
• Target species
  Mouse
• Tested applications
  Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
• Immunogen

  Mouse IgG whole molecule

• Conjugation
  TRITC. Ex: 547nm, Em: 572nm

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
• Concentration information loading...
• Purity
  IgG fraction
• Purification notes
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation notes
  Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

  • Secondary antibodies
  • anti-Mouse
  • IgG
  • Fluorophore
  • TRITC

Goat Anti-Mouse IgG H&L (TRITC) images

• 

  Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)
  Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.