Customer reviews and FAQs

Overview

  • Product name
    Goat Anti-Mouse IgG1 heavy chain
    See all IgG1 secondary antibodies
  • Target species
    Mouse
  • Specificity
    The anti IgG1 only reacts with IgG1 by IEP and gel diffusion analysis when tested against each of the mouse IgG subclasses at 1 mg/ml.
  • Tested applications
    Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details
  • Immunogen

    Goats were immunized with highly purified mouse IgG1 in Freunds adjuvant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS, pH7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The resulting antiserum was sequentially absorbed with IgG2a, IgG2b and IgG3 immunoabsorbants until it was IgG1 specific. The anti IgG1 was then immunoaffinity purified and tested for monospecificity.
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References for Goat Anti-Mouse IgG1 heavy chain (ab9165)

This product has been referenced in:
  • Fradkin AH  et al. UV photodegradation of murine growth hormone: Chemical analysis and immunogenicity consequences. Eur J Pharm Biopharm N/A:N/A (2014). Read more (PubMed: 24758742) »
  • Fradkin AH  et al. Glass particles as an adjuvant: a model for adverse immunogenicity of therapeutic proteins. J Pharm Sci 100:4953-64 (2011). Read more (PubMed: 21721003) »

See all 2 Publications for this product

Publishing research using ab9165? Please let us know so that we can cite the reference in this datasheet.

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2 Abreviews or Q&A

10
Votes: Highest First

You sell the following
http://www.abcam.com/Mouse-IgG-secondary-antibody-IgG1-Heavy-Chain-specific-ab9165.html
Is it available labeled for flow cytometery?

Thank you for contacting Abcam.

We do not currently supply ab9165 conjugated directly to a fluorphore, but we do have some kits that would allow you to do this yourself. These are our EasyLink conjugation kits:

http://www.abcam.com/...

Read More

Please send a protocol for using this mouse monoclonal antibody on mouse tissue.

Thank you for your enquiry. For staining mouse tissue with mouse monoclonal antibodies, please refer to the following two articles: Fung KM, Messing A, Lee VM, Trojanowski JQ. A novel modification of the avidin-biotin complex method for immunohisto...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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