Goat Anti-Mouse IgG1 heavy chain (ab97236)

Overview

  • Product nameGoat Anti-Mouse IgG1 heavy chain
    See all IgG1 secondary antibodies
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%.
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, ICCmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/30000. (Coating ELISA: 1/100 - 1/500, Primary ELISA: 1/1000-1/30000)
WB 1/1000 - 1/30000. (Chemiluminecent: 1/1000 - 1/30000, Colorimetric: 1/1000 - 1/10000)
IHC-P 1/200 - 1/2000.
ICC Use at an assay dependent dilution.

References for Goat Anti-Mouse IgG1 heavy chain (ab97236)

ab97236 has not yet been referenced specifically in any publications.

Product Wall

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Please check the following datasheets as answer to your first question;

http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

http://www.abcam.com/Go...

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The band size seems to be correct. This product has a predicted weight of 39kDa although you band seems a bit smaller this is common when using in real world settings as compared to predictions. Post translational...

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Thank you for contacting Abcam and thank you for sending the details of your protocol as well as the image. These have been very helpful.

After reviewing your protocol, I would like to suggest a few tips which may help reduce the upper dark ar...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this recommended product was not correct for your assay and for the inconvenience this has cau...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"