Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase) (ab97237)
Key features and details
- Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase)
See all IgG1 secondary antibodies -
Host species
Goat -
Target species
Mouse -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Conjugation notes
Molar enzyme: antibody protein ratio is 1:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/100 - 1/1000.
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ELISA |
1/1000 - 1/10000.
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WB |
1/1000 - 1/10000. Use chemiluminescence.
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/100 - 1/1000. |
ELISA
1/1000 - 1/10000. |
WB
1/1000 - 1/10000. Use chemiluminescence. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab97237 has been referenced in 4 publications.
- Thomas S et al. Development of a new fusion-enhanced oncolytic immunotherapy platform based on herpes simplex virus type 1. J Immunother Cancer 7:214 (2019). PubMed: 31399043
- Lynch JM et al. Binding of a glaucoma-associated myocilin variant to the aB-crystallin chaperone impedes protein clearance in trabecular meshwork cells. J Biol Chem 293:20137-20156 (2018). PubMed: 30389787
- Lynch JM et al. Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle. PLoS One 13:e0206801 (2018). PubMed: 30395621
- Aguilar-Rodríguez J et al. The Molecular Chaperone DnaK Is a Source of Mutational Robustness. Genome Biol Evol 8:2979-2991 (2016). PubMed: 27497316