Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase) (ab97237)

Overview

  • Product name
    Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase)
    See all IgG1 secondary antibodies
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notes
    Molar enzyme: antibody protein ratio is 1:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/10000. Use chemiluminescence.

References for Goat Anti-Mouse IgG1 heavy chain (Alkaline Phosphatase) (ab97237)

This product has been referenced in:
  • Aguilar-Rodríguez J  et al. The Molecular Chaperone DnaK Is a Source of Mutational Robustness. Genome Biol Evol 8:2979-2991 (2016). Read more (PubMed: 27497316) »

See 1 Publication for this product

Product Wall

We do have the same antibody conjugated with HRP: Goat Anti-Mouse IgG1 heavy chain (HRP) (http://www.abcam.com/ab97240)

Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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