Goat Anti-Mouse IgG1 heavy chain (HRP) (ab97240)

Overview

  • Product nameGoat Anti-Mouse IgG1 heavy chain (HRP)
    See all IgG1 secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (HRP)
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species.
  • Tested applicationsSuitable for: ICC, ELISA, IHC-P, WBmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
ELISA 1/10000 - 1/100000.
IHC-P 1/200 - 1/2000.
WB 1/2000 - 1/20000. Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method.

Goat Anti-Mouse IgG1 heavy chain (HRP) images

  • IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References for Goat Anti-Mouse IgG1 heavy chain (HRP) (ab97240)

This product has been referenced in:
  • Tor YS  et al. Induction of Apoptosis in MCF-7 Cells via Oxidative Stress Generation, Mitochondria-Dependent and Caspase-Independent Pathway by Ethyl Acetate Extract of Dillenia suffruticosa and Its Chemical Profile. PLoS One 10:e0127441 (2015). WB . Read more (PubMed: 26047480) »
  • Seth A  et al. Non-carrier nanoparticles adjuvant modular protein vaccine in a particle-dependent manner. PLoS One 10:e0117203 (2015). Read more (PubMed: 25756283) »

See all 13 Publications for this product

Product Wall

Application Western blot
This antibody is super specific! It does not react with mouse IgG2a or human IgG, or any IgG light chain I tested so far.

I use it because I work with mouse tumor lysate in western blot. All tumor lysate contains blood, so IgG is always in the lysates. When my protein of interest is at the same molecular weight of IgG light chain, I use this antibody.

The purpose of western blot I show here is to verify if this antibody reacts with light chain of mouse IgG1 specifically, so there is no primary antibody used.

The antibody is quite clean. The image I show is over-exposed with some background in order to confirm there is absolutely no IgG light chain signal.
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Verified customer

Submitted Oct 14 2015

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these t...

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Thank you for your enquiry.

I can confirm that ab43ERCC1 antibody [3H11] is a mouse monoclonal,IgG2a isotype.

The ab97240 secondary antibody detects mouse IgG1 isotype, so it would not be suitable for use with ab43. You will need ...

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Thank you very much for your email. I have compiled here below a list of antibodies which I think could correspond to your needs. There are three different IgG1 and three different IgG2a antibodies which have been tested against other isotypes. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"