Goat Anti-Mouse IgG2b heavy chain (HRP) (ab97250)

Overview

  • Product nameGoat Anti-Mouse IgG2b heavy chain (HRP)
    See all IgG2b secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgG2b - heavy chain (HRP)
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG2b. Cross reactivity with other immunoglobulins is less than 2%.
  • Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. Similar dilution to primary antibody.
WB 1/2000 - 1/25000. WB-Colorimetric 1/2000 - 1/20000; WB-Chemiluminescent 1/5000 - 1/25000

Goat Anti-Mouse IgG2b heavy chain (HRP) images

  • IHC image of Coilin staining in human testis formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab87913, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97250, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References for Goat Anti-Mouse IgG2b heavy chain (HRP) (ab97250)

This product has been referenced in:
  • Lee YL  et al. MAF1 represses CDKN1A through a Pol III-dependent mechanism. Elife 4:e06283 (2015). Read more (PubMed: 26067234) »
  • Soltanian S  et al. Expression analysis of BORIS during pluripotent, differentiated, cancerous, and non-cancerous cell states. Acta Biochim Biophys Sin (Shanghai) N/A:N/A (2014). Read more (PubMed: 24928684) »

See all 5 Publications for this product

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These products (ab97245, ab97250, ab98693) can be used as secondary antibodies.

Just let me know if you have further questions.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab98703.

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Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"